Cell Culture Techniques

Description

This 4-day workshop provides a solid basis for the understanding of the growth of animal cells and combines lecture and laboratory exercises aimed at giving participants a deeper understanding of serum-free media applications and related topics.

Topics include: Introduction to cell and tissue culture, serum containing media and the use of serum. cell counting and viability determination, development of growth curves, growth of adherent and suspension cells, transfection technologies for recombinant protein expression in animal cells, cryopreservation and cryo-thawing of animal cells, cell cloning and primary cell culture.

The workshop also contains sections on growth of cells in serum-free media, attachment, adaptation/selection for growth of adherent cells in suspension culture, the cell cycle and apoptosis as they relate to cells grown in culture.

The workshop is designed to provide a solid understanding of cell culture through classroom presentation and laboratory work. Clients will gain significant experience in the manipulation of animal cells through culture. Through integrated learning methods, utilizing hands-on training to reinforce lecture material participants will learn the biological basis and relevance of the process of culturing cells. This will allow clients to translate the techniques into applications in their own laboratories. At the end of the workshop we will conduct a full workshop review, and data troubleshooting session. This workshop provides prerequisite training for the culture of numerous cell types including stem cells.

Contact: Timothy Fawcett, Ph.D., Director
Email: tfawcett@btiworks.org